ABSTRACT
Objective: To identify Xanthii Fructus and secure its quality and safety in medication. Methods: Total ge-nomic DNA was extracted from Xanthii Fructus and its adulterants. ITS2 sequences were amplified, and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V 4.2. The Kimura 2-Parameter (K2P) distances were calculated using MEGA 5.0. The neigh-bor-joining (NJ) phylogenetic trees were constructed. Results: The intraspecific genetic distances of Xanthii Fructus were 0. The interspecific genetic distances between Xanthii Fructus and its adulterants were ranged from 0.009 to 0.542. The NJ tree showed that Xanthii Fructus could differ from its adulterants obviously. Conclusion: ITS2 can be used to identify Xanthii Fructus from its adulterants effectively, and our study further confirmed the effectiveness of ITS2 to identify traditional Chinese medicinal materials.
ABSTRACT
Objective: This study aimed to discriminate between Eupatorii Herba and its adulterants in order to guarantee the quality and clinical curative effect of this medicinal material. Methods: Genomic DNA extracted from Eupatorii Herba was used as templates. The internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA was amplified. Sequence assembly and consensus sequence generation were performed by CodonCode Aligner. The intraspecific and interspecific genetic distances of Eupatorii Herba and its adulterants were computed by MEGA5 and the phylogenetic tree was constructed using the neighbor-joining (NJ) method. Results: The length of ITS2 sequence of Eupatorii Herba was 218 bp. The maximum intraspecific genetic distance (K2P distance) of Eupatorii Herba was 0.0092. The minimum interspecific genetic distance of Eupatorii Herba and its adulterants was 0.024. The NJ trees showed that the ITS2 sequence would be used to identify Eupatorii Herba and its adulterants. Con-clusion: ITS2 sequence was able to identify Eupatorii Herba and its adulterants correctly and it provided a new technique to ensure clinical safety in utilization of traditional Chinese medicines.
ABSTRACT
Objective: To identify Persicae Semen and its sibling species, and to secure the quality and clinical safety of this medicinal material. Methods: The internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA of Persicae Semen and its sibling species were amplified and bidirectionally sequenced by DNA barcoding. Sequence assembly and consensus sequence generation were performed by the CodonCode Aligner 4.1. The genetic distances were computed by MEGA 5.0 in accordance with the Kimura 2-parameter (K2P) model, and the phylogenetic tree constructed by the neighbor-joining (NJ) method. Results: The length of ITS2 sequences of the two origin plants of Persicae Semen was between 212 bp to 213 bp. Their intraspecific genetic distance was much lower than the interspecific genetic distance with their sibling species. The ITS2 sequence possessed the character of good stability and low intra-specific sequence variation. In the NJ tree, both Prunus persica and P. davidiana were clustered into one large branch, and clearly separated with their sibling species. Conclusion: ITS2 can be used to effectively distinguish Persicae Semen from its sibling species, which can provide a reference for the iden-tification of other Chinese medicine and its sibling species.